CHOD-PAP Method Total Cholesterol Examination Validation on Samples Stored for 1 Week In 4-8°C Temperature
Jihan Nabilla
Published 2023-12-08
Keywords
- Total cholestrol,
- CHOD-PAP,
- Validation,
- Serum
Copyright (c) 2023 Jaringan Laboratorium Medis
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
CrossMark
Dimensions
If it doesn't Appear, click here
Impact Factor
Abstract
High total cholesterol levels cause various diseases, especially those related to the heart and blood vessels. Total cholesterol test uses serum as a sample and must be analyzed within one hour after sampling.However, in the field sometimes there are inspection delays.Some studies have proven that serum stored for one week at a temperature of 4-8°C has different results in total cholesterol examination. For this reason, this study aims to determine the validity of total cholesterol examination using the CHOD-PAP method of serum samples stored for one week at a temperature of 4o-8oC. Validation test based on eight parameters, there are precision, accuracy, linearity, reportable range,limit detection, quantitation limit, recovery, and interference. Only the recovery test was not accepted from eight parameters. Based on the Kruskal Wallis test, the sig value is 0.172 <0.05, it can be stated that there is no difference in serum storage time for one week at a temperature of 4-8℃ on total cholesterol levels using CHOD-PAP method.
Downloads
References
- Hartini, Supri, and Maria Eka Suryani. (2016). “Uji Kualitas Serum Simpanan Terhadap Kadar Kolesterol Dalam Darah Di Poltekkes Kemenkes Kaltim.” Jurnal Ilmiah Manuntung 2(1):65–69. https://doi.org/10.51352/jim.v2i1.49
- Hidayat, A. Aziz Alimul. 2010. “Metode Penelitian Kesehatan Paradigma Kuantitatif,” Health Book Publishing Surabaya
- Kamilla, Laila, and Slamet. (2017). “Pengaruh Lamanya Penyimpanan Serum Pada Suhu 2o -8o C Selama Satu Minggu Terhadap Kadar Kolesterol Total.” Jurnal Laboratorium Khatulistiwa 1(1):17–20. https://doi.org/10.30602/jlk.v1i1.90
- Kee, Joyce LeFever. (2008). “Pedoman Pemeriksaan Laboratorium & Diagnostik Edisi ke-6,” EGC Jakarta.
- Little, Thomas A. (2016). Establishing Acceptance Criteria for Analytical Methods.
- Merdekawati, Fusvita, and Nani Kurnaeni. (2019). “Reagen Buatan Sendiri Sebagai Reagen Alternatif Untuk Pemeriksaan Kadar Albumin.” Meditory : The Journal of Medical Laboratory 7(2):77–93. http://ejournal.poltekkes-denpasar.ac.id/index.php/M
- Mulyati, Ade Heri, Sutanto, and Dewi Apriyani. (2011). “Validasi Metode Analisis Kadar Ambroksol Hidroklorida Dalam Sediaan Tablet Cystelis® Secara Kromatografi Cair Kinerja Tinggi.” Ekologia 11(2):36–45. https://journal.unpak.ac.id/index.php/ekologia/article/view/259
- Nurhayati, Ika, Ani Riyani, Nani Kurnaeni, Wiwin Wiryanti, and Sonny Feisal Rinaldi. (2019). “Validasi Metode God-Pap Pada Pemeriksaan Glukosa Darah Dengan Pemakaian Setengah Volume Reagen Dan Sampel.” Jurnal Riset Kesehatan Poltekkes Depkes Bandung 11(1):322–36. https://doi.org/10.34011/juriskesbdg.v11i1.792
- Riyanto, Ph. D. (2014). “Validasi & Verifikasi Metode Uji Sesuai Dengan ISO/IEC 17025 Laboratorium Pengujian Dan Kalibrasi.” 1–154. https://www.scribd.com/document/442255168/Validasi-dan-Verifikasi-Metode-Uji-Sesuai-dengan-ISO-atau-IEC-17025-Laboratorium-Pengujian-dan-Kalibrasi
- Subrata Tri Widada, M. Atik Martisiningsik, Stephanie Cicilia Carolina. (2016). “Gambaran Perbedaan Kadar Kolesterol Total Metode CHOD - PAP (Cholesterol Oxidase - Peroxidase Aminoantypirin) Sampel Serum Dan Sampel Plasma EDTA.” Jurnal Teknologi Laboratorium 5(1):41–44. https://www.teknolabjournal.com/index.php/Jtl/article/view/76/55